Bacteria and Fungi
Alcian Yellow - Toluine Blue for Helicobactor Pylori
Integrated method to show Helicobacter pylori and acid mucins in gastric tissue sections.
Brown-Brenn
For demonstration Gram-positive and Gram-negative bacteria
Giemsa for Helicobacter Pylori
To demonstrate Helicobacter pylori bacterium on gastric biopsies.
Gram
To differentiate between Gram-positive and Gram-negative bacteria in tissue sections and smears.
Grocott
To demonstrate fungi in tissue sections.
Grocott Using Microwave
To demonstrate fungi in tissue sections.
May Grunwald Giemsa
Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue and to demonstrate endothelial reticulum.
Warthin-Starry
Method for the demonstration of spirochetes.
Ziehl-Neelsen
To show pathogenic mycobacteria (especially Koch’s bacillus) in histologic sections, sputum smears and culture smears
Ziehl Neelsen, Fite
To show pathogenic mycobacteria (especially Koch’s bacillus and Hansen’s bacillus) in histologic sections, sputum smears and culture smears.
Ziehl-Neelsen for Cryptosporidium
To show Cryptosporidium sp. oocysts in faecal smears.
Special Staining Kits
- Ready to use
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- Reproducible results with full protocol
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- Minimal acceptance testing
Carbohydrates
Alcian Blue pH 2.5
For demonstration of the acid mucopolysaccharides in tissue sections.
Alcian Blue pH 2.5 PAS
Integrated method to show acid mucins, neutral mucins and carbohydrates in tissue sections.
Colloidial Iron
For demonstration of acid mucins which form a stable complex with trivalent iron thanks to the presence of their acid groups in anionic form.
Congo Red
Method to show amyloid in tissue sections.
Enzymatic Digestion, Amylase
Removal of glycogen from tissue sections
Enzymatic Digestion, Diastase
Removal of glycogen in order to observe epithelial neutral mucins. This method is recommended for liver biopsy.
PAS
To demonstrate normal and pathologic tissue components characterised by adjacent glycolic or aminohydroxylic groups.
Sirius Red
Method to show amyloid in tissue sections.
Sirius Red Picrate
For demonstration of collagen fibres and bilirubin in tissue sections.
Collagen and Connective Tissue
Afog - Acid Fuchsin Orange G
Standard procedure for visualisation of glomerular protein deposits in kidney biopsies.
Azan Trichrome
Method for connective tissue, especially to show muscle fibres, glial fibres, collagen, reticulum, kidney glomerular stroma, erythrocytes and nuclear chromatin
Gomori Fuchsin Paraldehyde
For demonstration elastic fibres, beta cells of pancreas, mast cells granules, mucin, argentaffin granules and acrosomes of spermatozoa.
Gordon-Sweet for Reticulum Fibres
Recommended method to show argyrophilic reticular fibres in connective tissue.
Mallory Trichrome
Procedure for showing connective tissue including collagen, reticulum, cartilage, bone and amyloid.
Masson Trichrome
Method for connective tissue demonstrating gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus.
Masson Trichrome - Goldner
Method for connective tissue showing gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus. Suitable for black and white microphotographs.
Orcein
To demonstrate elastic fibres in tissue sections, especially in vascular studies.
Perls Van Gieson
To show ferric iron, collagen and connective tissue.
Phosfotungstic Acid Haematoxylin
Used to differentiate between smooth and striated muscular tissue by staining isotropic bands of skeletal muscle myofibrils and to show fibrin.
Picro Mallory
Trichrome stain recommended for connective sections.
Silver Impregnation
Recommended method to show argyrophilic reticular fibres in connective tissue, especially to differentiate collagen fibres from connective tissue.
Silver Methenamine
To show argyrophilic elements and mucopolysaccharides (basal membranes, mycetes, bacteria etc.) in tissue sections. Recommended method to examine basal membrane in renal biopsy.
Van Gieson, Trichrome
Recommended method for connective tissue, especially to differentiate collagen fibres from connective tissue.
Verhoeff
To demonstrate elastic fibres in tissue sections, especially in vascular pathology
Weigert, Long Method
To demonstrate elastic fibres in tissue sections.
Weigert, Rapid Method
To demonstrate elastic fibres in tissue sections. Recommended for vascular pathology.
Weigert Van Gieson, Long Method
To demonstrate elastic fibres, connective tissue, collagen and nuclei.
Weigert Van Gieson, Rapid Method
To show elastic fibres, connective tissue and nuclei at the same time. Recommended for vascular pathology.
Cytology
May Grunwald Giemsa
Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue and to demonstrate endothelial reticulum.
MGG Quick Stain
For differential staining of formed blood elements. It can also be used to stain other types of air-dried smears such as sediment smears and needle aspirates. The staining can be compared to traditional May Grunwald-Giemsa method.
Special Staining Kits
- Ready to use
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- Reproducible results with full protocol
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- Minimal acceptance testing
Lipids
Oil Red O
Recommended method for demonstration of lipids on frozen sections.
Nervous Tissue
Bielschowsky
Recommended method to show neurofibrils, axons, dendrites and senile plaques in Alzheimer's disease.
Luxol Fast Blue Kluwer Barrera
To show myelin and phospholipids in histologic sections.
Special Staining Kits
- Ready to use
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- Reproducible results with full protocol
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- Minimal acceptance testing
Nuclei
AgNOR
To show argentaffine proteins (100 KD), which are present in NOR region, in smears and sections embedded in paraffin.
Pigments and Mineral Deposits
Masson Fontana
Recommended method to show melanin.
Perls
To show reactive ferric iron.
Perls Van Gieson
To show ferric iron, collagen and connective tissue.
Sirius Red
Method to show amyloid in tissue sections.
Sirius Red Picrate
For demonstration of collagen fibres and bilirubin in tissue sections.
Van Gieson, Fouchet
For demonstration of bilirubin and collagen in tissue sections
Von Kossa
To demonstrate deposits of calcium ions in tissue sections.
Enzyme Histochemistry
Acid Phosphatase
To show the enzymatic activity of acid phosphatase. Acid phosphatase is in macrophage and lysosome, it identifies necrosis and regeneration.
Alkaline Phosphatase
Evaluation of alkaline phosphatase activity. Alkaline phosphatase is particularly useful in revealing sites of phagocytosis and inflammation in muscle biopsy specimens.
ATPase
Typing of muscle fibres.
Cytochrome C Oxidase
Activity evaluation of Cytochrome C oxidase
Esterase
Histochemical demonstration of esterase in denervated fibres.
Myoadenylate Deaminase
To show the enzymatic activity of Myoadenylate deaminase
NADH Diaphorase
For distinguishing type 1 and type 2 muscle fibres. Often it is correlated with the ATPase procedure.
PFK - Phosphofructokinase
To show PFK enzymatic activity. This procedure is used to determine if a glycogen-storage disease is due to a lack of PFK activity or about other enzymes necessary for the metabolism of glycogen.
Phosphorylase
Histochemical demonstration of phosphorylase.
Succinic Dehydrogenase
Evaluation of the succinic dehydrogenase activity specifically in mitochondria.