Special Staining Kits

Phosfotungstic Acid Haematoxylin

Used to differentiate between smooth and striated muscular tissue by staining isotropic bands of skeletal muscle myofibrils and to show fibrin.

Fast Papanicolaou

A highly selective blue nuclear stain, Harris’ haematoxylin, is combined with IW3 polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from eosinophil.

Acetylcholinesterase

Acetylcholinesterase staining kit shows the presence and distribution of the colinergic fibres. 

Afog - Acid Fuchsin Orange G

Standard procedure for visualisation of glomerular protein deposits in kidney biopsies. 

AgNOR

To show argentaffine proteins (100 KD), which are present in NOR region, in smears and sections embedded in paraffin.

Alcian Blue pH 0.5

For demonstration of the very strongly sulphured mucins in tissue sections.

Alcian Blue pH 1.0

Method to show the strongly sulphured mucins only in tissue sections.

Alcian Blue pH 2.5

For demonstration of the acid mucopolysaccharides in tissue sections.

Alcian Blue pH 2.5 PAS

Integrated method to show acid mucins, neutral mucins and carbohydrates in tissue sections.

Azan Trichrome

Method for connective tissue, especially to show muscle fibres, glial fibres, collagen, reticulum, kidney glomerular stroma, erythrocytes and nuclear chromatin

Alcian Yellow - Toluine Blue for Helicobactor Pylori

Integrated method to show Helicobacter pylori and acid mucins in gastric tissue sections.

Brown-Brenn

For demonstration Gram-positive and Gram-negative bacteria

Bielschowsky

Recommended method to show neurofibrils, axons, dendrites and senile plaques in Alzheimer's disease.

Congo Red

Method to show amyloid in tissue sections.

Colloidial Iron

For demonstration of acid mucins which form a stable complex with trivalent iron thanks to the presence of their acid groups in anionic form.

Dane

For demonstration of pre-keratin, keratin and mucin. Particularly indicated in skin pathology.

Enzymatic Digestion, Amylase

Removal of glycogen from tissue sections

Enzymatic Digestion, Diastase

Removal of glycogen in order to observe epithelial neutral mucins. This method is recommended for liver biopsy.

Enzymatic Digestion, Hyaluronidase

For the removal of carbohydrates: hyaluronic acid, chondroitin sulphate (A and B).

Feulgen

Demonstration of DNA in tissue sections.

Giemsa for Helicobacter Pylori

To demonstrate Helicobacter pylori bacterium on gastric biopsies.

Gomori Fuchsin Paraldehyde

For demonstration elastic fibres, beta cells of pancreas, mast cells granules, mucin, argentaffin granules and acrosomes of spermatozoa.

Gordon-Sweet for Reticulum Fibres

Recommended method to show argyrophilic reticular fibres in connective tissue.

Gram

To differentiate between Gram-positive and Gram-negative bacteria in tissue sections and smears.

Grimelius

To show argyrophilic substances in tissue sections.

Grocott

To demonstrate fungi in tissue sections.

Grocott Using Microwave

To demonstrate fungi in tissue sections.

Luxol Fast Blue Kluwer Barrera

To show myelin and phospholipids in histologic sections.

Mallory Trichrome

Procedure for showing connective tissue including collagen, reticulum, cartilage, bone and amyloid.

Masson Fontana

Recommended method to show melanin.

Masson Trichrome

Method for connective tissue demonstrating gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus.

Masson Trichrome - Goldner

Method for connective tissue showing gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus. Suitable for black and white microphotographs.

May Grunwald Giemsa

Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue and to demonstrate endothelial reticulum.

Methyl Green Pyronin

To demonstrate simultaneously DNA and RNA in histologic sections. Recommended to show plasma cells and RNA in histologic sections and cytological preparations.

Mucicarmine

To show acid mucopolysaccharides of epithelial origin (mucins) in histologic sections

Oil Red O

Recommended method for demonstration of lipids on frozen sections.

Orcein

To demonstrate elastic fibres in tissue sections, especially in vascular studies.

PAS - Morel Maronger

For the demonstration of tissue components characterised by adjacent glycol or aminohydroxylic groupings and connective tissue.

PAS - Pearse

P.A.S. reaction particularly designed to demonstration of alpha and beta cells on the pituitary gland.

PAS

To demonstrate normal and pathologic tissue components characterised by adjacent glycolic or aminohydroxylic groups. 

Perls Van Gieson

To show ferric iron, collagen and connective tissue.

Picro Mallory

Trichrome stain recommended for connective sections.

Rapid Frozen Section

Rapid H&E staining method for frozen sections

Silver Impregnation

Recommended method to show argyrophilic reticular fibres in connective tissue, especially to differentiate collagen fibres from connective tissue.

Silver Methenamine

To show argyrophilic elements and mucopolysaccharides (basal membranes, mycetes, bacteria etc.) in tissue sections. Recommended method to examine basal membrane in renal biopsy.

Sirius Red

Method to show amyloid in tissue sections.

Sirius Red Picrate

For demonstration of collagen fibres and bilirubin in tissue sections.

Van Gieson, Fouchet

For demonstration of bilirubin and collagen in tissue sections

Van Gieson, Trichrome

Recommended method for connective tissue, especially to differentiate collagen fibres from connective tissue.

Verhoeff

To demonstrate elastic fibres in tissue sections, especially in vascular pathology

Von Kossa

To demonstrate deposits of calcium ions in tissue sections.

Warthin-Starry

Method for the demonstration of spirochetes.

Weigert, Rapid Method

To demonstrate elastic fibres in tissue sections. Recommended for vascular pathology.

Weigert, Long Method

To demonstrate elastic fibres in tissue sections.

Weigert Van Gieson, Rapid Method

To show elastic fibres, connective tissue and nuclei at the same time. Recommended for vascular pathology.

Weigert Van Gieson, Long Method

To demonstrate elastic fibres, connective tissue, collagen and nuclei.

Wilson's Disease for Copper

To demonstrate copper in hepatic tissue sections.

Ziehl-Neelsen

To show pathogenic mycobacteria (especially Koch’s bacillus) in histologic sections, sputum smears and culture smears

Ziehl Neelsen, Fite

To show pathogenic mycobacteria (especially Koch’s bacillus and Hansen’s bacillus) in histologic sections, sputum smears and culture smears.

Ziehl-Neelsen for Cryptosporidium

To show Cryptosporidium sp. oocysts in faecal smears.

MGG Quick Stain 

For differential staining of formed blood elements. It can also be used to stain other types of air-dried smears such as sediment smears and needle aspirates. The staining can be compared to traditional May Grunwald-Giemsa method.

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