Special Staining Kits
Afog - Acid Fuchsin Orange G
Standard procedure for visualisation of glomerular protein deposits in kidney biopsies.
AgNOR
To show argentaffine proteins (100 KD), which are present in NOR region, in smears and sections embedded in paraffin.
Alcian Blue pH 2.5 PAS
Integrated method to show acid mucins, neutral mucins and carbohydrates in tissue sections.
Alcian Blue pH 2.5
For demonstration of the acid mucopolysaccharides in tissue sections.
Azan Trichrome
Method for connective tissue, especially to show muscle fibres, glial fibres, collagen, reticulum, kidney glomerular stroma, erythrocytes and nuclear chromatin
Alcian Yellow - Toluine Blue for Helicobactor Pylori
Integrated method to show Helicobacter pylori and acid mucins in gastric tissue sections.
Brown-Brenn
For demonstration Gram-positive and Gram-negative bacteria
Bielschowsky
Recommended method to show neurofibrils, axons, dendrites and senile plaques in Alzheimer's disease.
Colloidial Iron
For demonstration of acid mucins which form a stable complex with trivalent iron thanks to the presence of their acid groups in anionic form.
Enzymatic Digestion, Amylase
Removal of glycogen from tissue sections
Congo Red
Method to show amyloid in tissue sections.
Enzymatic Digestion, Diastase
Removal of glycogen in order to observe epithelial neutral mucins. This method is recommended for liver biopsy.
Fast Papanicolaou
A highly selective blue nuclear stain, Harris’ haematoxylin, is combined with IW3 polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from eosinophil.
Giemsa for Helicobacter Pylori
To demonstrate Helicobacter pylori bacterium on gastric biopsies.
Gomori Fuchsin Paraldehyde
For demonstration elastic fibres, beta cells of pancreas, mast cells granules, mucin, argentaffin granules and acrosomes of spermatozoa.
Gordon-Sweet for Reticulum Fibres
Recommended method to show argyrophilic reticular fibres in connective tissue.
Gram
To differentiate between Gram-positive and Gram-negative bacteria in tissue sections and smears.
Grocott
To demonstrate fungi in tissue sections.
Grocott Using Microwave
To demonstrate fungi in tissue sections.
Luxol Fast Blue Kluwer Barrera
To show myelin and phospholipids in histologic sections.
Mallory Trichrome
Procedure for showing connective tissue including collagen, reticulum, cartilage, bone and amyloid.
Masson Fontana
Recommended method to show melanin.
Masson Trichrome
Method for connective tissue demonstrating gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus.
Masson Trichrome - Goldner
Method for connective tissue showing gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus. Suitable for black and white microphotographs.
May Grunwald Giemsa
Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue and to demonstrate endothelial reticulum.
MGG Quick Stain
For differential staining of formed blood elements. It can also be used to stain other types of air-dried smears such as sediment smears and needle aspirates. The staining can be compared to traditional May Grunwald-Giemsa method.
Mucicarmine
To show acid mucopolysaccharides of epithelial origin (mucins) in histologic sections
Oil Red O
Recommended method for demonstration of lipids on frozen sections.
Orcein
To demonstrate elastic fibres in tissue sections, especially in vascular studies.
PAS
To demonstrate normal and pathologic tissue components characterised by adjacent glycolic or aminohydroxylic groups.
Phosfotungstic Acid Haematoxylin
Used to differentiate between smooth and striated muscular tissue by staining isotropic bands of skeletal muscle myofibrils and to show fibrin.
Perls Van Gieson
To show ferric iron, collagen and connective tissue.
Picro Mallory
Trichrome stain recommended for connective sections.
Rapid Frozen Section
Rapid H&E staining method for frozen sections
Silver Impregnation
Recommended method to show argyrophilic reticular fibres in connective tissue, especially to differentiate collagen fibres from connective tissue.
Silver Methenamine
To show argyrophilic elements and mucopolysaccharides (basal membranes, mycetes, bacteria etc.) in tissue sections. Recommended method to examine basal membrane in renal biopsy.
Sirius Red
Method to show amyloid in tissue sections.
Sirius Red Picrate
For demonstration of collagen fibres and bilirubin in tissue sections.
Van Gieson, Fouchet
For demonstration of bilirubin and collagen in tissue sections
Van Gieson, Trichrome
Recommended method for connective tissue, especially to differentiate collagen fibres from connective tissue.
Verhoeff
To demonstrate elastic fibres in tissue sections, especially in vascular pathology
Von Kossa
To demonstrate deposits of calcium ions in tissue sections.
Warthin-Starry
Method for the demonstration of spirochetes.
Weigert, Rapid Method
To demonstrate elastic fibres in tissue sections. Recommended for vascular pathology.
Weigert, Long Method
To demonstrate elastic fibres in tissue sections.
Weigert Van Gieson, Rapid Method
To show elastic fibres, connective tissue and nuclei at the same time. Recommended for vascular pathology.
Weigert Van Gieson, Long Method
To demonstrate elastic fibres, connective tissue, collagen and nuclei.
Ziehl-Neelsen
To show pathogenic mycobacteria (especially Koch’s bacillus) in histologic sections, sputum smears and culture smears
Ziehl Neelsen, Fite
To show pathogenic mycobacteria (especially Koch’s bacillus and Hansen’s bacillus) in histologic sections, sputum smears and culture smears.
Ziehl-Neelsen for Cryptosporidium
To show Cryptosporidium sp. oocysts in faecal smears.
